A549 is an adenocarcinomic cell line that was established in 1972 from the lung tissue of a 58-year-old Caucasian male. A549 is categorized as a non-small-cell lung carcinoma, which is more common yet less aggressive than small cell lung carcinoma. A549 cells are hypotriploid, have a modal chromosome number of 66, and exhibit an epithelial-like morphology with adherent cultural properties. A549 cells are suitable transfection hosts for in vivo and in vitro models, and an A549 Transfection Reagent can be found at Altogen Biosystems . An A549 xenograft model drawn from an in vivo transfection can be found here . The A549 cell line widely serves as a model for the study of not only lung adenocarcinoma, but also respiratory infections, asbestos exposure, and viral infections stemming from asthma.
In the following study, researchers investigate the cytotoxic effects of silver nitrate on A549 cells. MTT assay, flow cytometry, immunocytochemical, confocal and transmission electron microscopy, and microarray assays were used to analyze the effects of silver nitrate. Results show that silver nitrate exhibit inhibitory effects against A549 cells in a dose- and time-dependent manner, ultimately inducing apoptosis. These results suggest that silver nitrate may be used as an effective therapeutic agent against lung cancer. [ LINK ]