Having been studied extensively over the past few decades, A549 cells have become one of the major players in the lung carcinoma field. With numerous applications in cell signaling studies and cancer oncogene evaluations, A549 xenografts have become prime targets for transfection experiments and related research. The cells are set apart by their ability to synthesize lecithin and their internal environment; a high level of unsaturated fatty acids that contribute to the maintenance of membrane phospholipids in lung epithelial cells. The cell line has been used extensively as an in vitro host capable of receiving nucleic acids and responding effectively in drug metabolism studies.
The A549 cell line was established in 1972 by DJ Giad, et al. The original cells were derived from a cell culture of carcinomatous lung tissue of a 58 year old Caucasian male with lung cancer. Additional examination of the cells by M. Lieber, et. al. revealed the cells could synthesize lecithin with a higher percentage of denaturated fatty acid through the cytidine diphosphocholine pathway. The cells are hypotriploid human cells with 24% containing a modal chromosome number of 66. Findings have frequently found that the cells have a chromosomal count of 64,65,or 67. Typically, these cells act as in vitro cell models for type II pulmonary epithelial cell research examining drug metabolism within the cell. Often the A549 cells are used for research studies on respiratory illnesses such as, viral induced asthma infections, lung tissue damage resulting from asbestos exposure and smoker-related emphysema. The development of knockdown systems and gene the...
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