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Altogen Biosystems - Provider of Optimized Transfection Reagents


Altogen Biosystems is a well-known provider of transfection reagents and laboratory services world-wide for researchers in a variety of fields. Biology contract research services and products that are offered include assay development, reliable 28-day generation of stably-transfected cell lines, and _ laboratory services such as siRNA screening and ELISA assay development. For an A549 cell xenograftmodel, visit the Altogen Biosystems website.

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A549 Cell Line

A549 is an adenocarcinomic cell line that was established in 1972 from the lung tissue of a 58-year-old Caucasian male. A549 is categorized as a non-small-cell lung carcinoma, which is more common yet less aggressive than small cell lung carcinoma. A549 cells are hypotriploid, have a modal chromosome number of 66, and exhibit an epithelial-like morphology with adherent cultural properties. A549 cells are suitable transfection hosts for  in vivo  and  in vitro  models, and an A549 Transfection Reagent can be found at Altogen Biosystems . An A549 xenograft model drawn from an  in vivo  transfection can be found here . The A549 cell line widely serves as a model for the study of not only lung adenocarcinoma, but also respiratory infections, asbestos exposure, and viral infections stemming from asthma.
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Side Population Cells Present in A549 Cells

In the following study, researchers isolate and characterize side population (SP) cells that are present in the A549 cancer cell line. Fluorescence-activated cell sorting, assays, and a chemoresistance analysis were used to isolate and differentiate SP and non-SP (NSP) cells. Results show that SP cells account for 1.09% of live A549 cells and produced both SP and NSP cells. SP cells also exhibited improved invasive ability and had higher levels of chemoresistance compared to NSP cells in vitro.  Therefore, SP cells isolated from the A549 cell line exhibited more tumorigenicity, along with invasive ability and chemoresistance, than NSP cells. [ LINK ]
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A549 Transfection Reagent

The A549 cell line was established in 1972 by DJ Giad, et al. The original cells were derived from a cell culture of carcinomatous lung tissue of a 58 year old Caucasian male with lung cancer. Additional examination of the cells by M. Lieber, et. al. revealed the cells could synthesize lecithin with a higher percentage of denaturated fatty acid through the cytidine diphosphocholine pathway. The cells are  hypotriploid human cells with 24% containing a modal chromosome number of 66. Findings have frequently found that the cells have a chromosomal count of  64,65,or 67. Typically, these cells act as  in vitro  cell models for type II pulmonary epithelial cell research examining drug metabolism within the cell. Often the A549  cells are used for research studies on respiratory illnesses such as, viral induced asthma infections, lung tissue damage resulting from asbestos exposure and smoker-related emphysema.  The development of knockdown systems and gene the...
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