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A549 Transfection Reagent

The A549 cell line was established in 1972 by DJ Giad, et al. The original cells were derived from a cell culture of carcinomatous lung tissue of a 58 year old Caucasian male with lung cancer. Additional examination of the cells by M. Lieber, et. al. revealed the cells could synthesize lecithin with a higher percentage of denaturated fatty acid through the cytidine diphosphocholine pathway. The cells are hypotriploid human cells with 24% containing a modal chromosome number of 66. Findings have frequently found that the cells have a chromosomal count of  64,65,or 67. Typically, these cells act as in vitro cell models for type II pulmonary epithelial cell research examining drug metabolism within the cell. Often the A549 cells are used for research studies on respiratory illnesses such as, viral induced asthma infections, lung tissue damage resulting from asbestos exposure and smoker-related emphysema. 

The development of knockdown systems and gene therapy treatments is possible through the use of the A549 transfection reagent developed by Altogen BiosystemsThis transfection reagent enables the delivery of small interfering (siRNA), messenger RNA (mRNA), pDNA, and miRNA with an 80% efficiency rate. The A549 reagent has been pre-optimized to work efficiently in serum for consitent and easily reproducible studies. 

A549 Transfection Reagent (Lung Carcinoma, CCL-185)

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Silver Nitrate Induces Apoptosis in A549 Cell Line

In the following study, researchers investigate the cytotoxic effects of silver nitrate on A549 cells. MTT assay, flow cytometry, immunocytochemical, confocal and transmission electron microscopy, and microarray assays were used to analyze the effects of silver nitrate. Results show that silver nitrate exhibit inhibitory effects against A549 cells in a dose- and time-dependent manner, ultimately inducing apoptosis. These results suggest that silver nitrate may be used as an effective therapeutic agent against lung cancer. [ LINK ]

A549 Cell Line

A549 is an adenocarcinomic cell line that was established in 1972 from the lung tissue of a 58-year-old Caucasian male. A549 is categorized as a non-small-cell lung carcinoma, which is more common yet less aggressive than small cell lung carcinoma. A549 cells are hypotriploid, have a modal chromosome number of 66, and exhibit an epithelial-like morphology with adherent cultural properties. A549 cells are suitable transfection hosts for  in vivo  and  in vitro  models, and an A549 Transfection Reagent can be found at Altogen Biosystems . An A549 xenograft model drawn from an  in vivo  transfection can be found here . The A549 cell line widely serves as a model for the study of not only lung adenocarcinoma, but also respiratory infections, asbestos exposure, and viral infections stemming from asthma.